Developmental Effects of Amoxicillin at Environmentally Relevant Concentration Using Zebrafish Embryotoxicity Test (ZET)

Amoxicillin (AMX) is an antibiotic that has been added to the watch list of substances by European Union, through Water Framework Directive (WFD; 2000/60/EC) and its daughter regulation (Decision 2018/840) for which monitoring data have be gathered with a possibility future regulation. Previous studies demonstrated this generates toxic effects, among oxidative stress in aquatic organisms noteworthy. The aim study was evaluate effect on embryonic development teratogenic effects induced AMX at environmentally relevant concentrations oocytes embryos Danio rerio. Furthermore, biomarkers were evaluated 72 96 hpf. LC50 14.192 μgL−1, EC50 7.083 TI 2.003. Biomarkers cellular oxidation antioxidant enzymes modified concentration-dependent way respect control group (p<0.05). main malformations identified tail malformation, pericardial edema, yolk sac scoliosis, pectoral fin absence, no hatching. results allow us conclude capable inducing embryotoxic damage. This compound represents risk such as Not applicable.